Chondrogenic differentiation in the context of the growth plate development or osteochondral pathologies is a process that is thoroughly regulated with inputs from multiple regulators active at multiple time/length scales. A previously developed intracellular regulatory model of chondrogenic differentiation will be extended to integrate the secretome data obtained in the CARBON project with an interactive approach. We will aim to understand the interplay between different biological components and biomechanical factors in the onset and progression of endochondral ossification.
Additionally, specific attention will be paid to the influence of extracellular matrix, and mechanics on the regulatory network dynamics. Finally, The ultimate goal of this project is the development of a tool that can be used both to investigate fundamental questions on osteoarthritis and bone healing but also to suggest potential therapeutic strategies that might be used for inhibition of osteoarthritis or for stimulation of bone repair.